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Image Search Results
Journal: eLife
Article Title: The m 6 A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells
doi: 10.7554/eLife.75827
Figure Lengend Snippet:
Article Snippet: Antibody ,
Techniques: Recombinant, Plasmid Preparation, Sequencing, shRNA, Negative Control, Mutagenesis, Bicinchoninic Acid Protein Assay, Transfection, Immunoprecipitation, Software, Modification, Saline
Journal: Neural Regeneration Research
Article Title: Reperfusion after hypoxia-ischemia exacerbates brain injury with compensatory activation of the anti- ferroptosis system: based on a novel rat model
doi: 10.4103/1673-5374.369117
Figure Lengend Snippet: Immunofluorescence staining and Western blot analysis of the different groups. (A, B) Immunofluorescence staining for glial fibrillary acidic protein (GFAP, FITC) and myelin basic protein (MBP, Cy3) expression in the different groups. Higher GFAP expression and lower MBP expression were observed in the HIRBI group compared to Sham group. Scale bars: 100 μm. (C) Detection of microtubule-associated protein 2 (MAP2), GFAP, MBP, Ferritin, and glutathione peroxidase 4 (GPX4) expression by western blot assay. (D) Quantification of western blot bands (normalized to β-actin). Higher GFAP, GPX4, and Ferritin expression and lower MAP2 and MBP expression were observed in the HIRBI group compared to Rice-Vannucci group. Data are expressed as mean ± SD ( n = 3). * P < 0.05 (one-way analysis of variance followed by Tukey’s honestly significant difference test).
Article Snippet: After washing with Tris-buffered saline (Sigma) and Tris-buffered saline-Tween (Thermo Fisher Scientific), the polyvinylidene fluoride membranes were incubated with primary antibodies to
Techniques: Immunofluorescence, Staining, Western Blot, Expressing